High-throughput RNAi screening in vitro: From cell lines to primary cells
نویسندگان
چکیده
منابع مشابه
High-throughput RNAi screening in vitro: from cell lines to primary cells.
Small interfering RNAs (siRNAs) are being used to induce sequence-specific gene silencing in cultured cells to study mammalian gene function. Libraries of siRNAs targeting entire human gene classes can be used to identify genes with specific cellular functions. Here we describe high-throughput siRNA delivery methods to facilitate siRNA library screening experiments with both immortalized and pr...
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ژورنال
عنوان ژورنال: RNA
سال: 2005
ISSN: 1355-8382
DOI: 10.1261/rna.7288405